AgrobacTransV1, Main, Exploration, bibRecord, 000858

Transgenic resistance in potato plants expressing potato leaf roll virus (PLRV) replicase gene sequences is RNA-mediated and suggests the involvement of post-transcriptional gene silencing.

Identifieur interne : 000858 ( Main/Exploration ); précédent : 000857; suivant : 000859

Transgenic resistance in potato plants expressing potato leaf roll virus (PLRV) replicase gene sequences is RNA-mediated and suggests the involvement of post-transcriptional gene silencing.

Auteurs : C. Vazquez Rovere [Argentine] ; S. Asurmendi ; H E Hopp

Source :

Archives of virology [ 0304-8608 ] ; 2001.

RBID : pubmed:11556710

Descripteurs français

English descriptors

KwdEn :
- Cloning, Molecular (MeSH), Gene Silencing (MeSH), Luteovirus (enzymology), Luteovirus (genetics), Molecular Sequence Data (MeSH), Open Reading Frames (MeSH), Plants, Genetically Modified (virology), RNA Replicase (genetics), Sequence Homology, Nucleic Acid (MeSH), Solanum tuberosum (genetics), Solanum tuberosum (virology), Transformation, Genetic (MeSH).
MESH :
- chemical , genetics : RNA Replicase.
- enzymology : Luteovirus.
- genetics : Luteovirus, Solanum tuberosum.
- virology : Plants, Genetically Modified, Solanum tuberosum.
- Cloning, Molecular, Gene Silencing, Molecular Sequence Data, Open Reading Frames, Sequence Homology, Nucleic Acid, Transformation, Genetic.

Abstract

Genetically engineered expression of replicase encoding sequences has been proposed as an efficient system to confer protection against virus diseases by eliciting protection mechanisms in the plant. Potato leaf-roll was one of the first diseases for which this kind of protection was engineered in potato plants. However, details of the protecting mechanism were not reported, so far. The ORF2b of an Argentinean strain of PLRV was cloned and sequenced finding 94% and 97% of homology with Australian and Dutch strains, respectively. To elucidate the mechanism of protection against PLRV infection, three versions of ORF2b (non-translatable sense, translatable sense with an engineered ATG and antisense) were constructed under the control of the 35S CaMV promoter and the nos terminator and introduced in potato plants (cv. Kennebec) by Agrobacterium tumefaciens-mediated transformation. Grafting infection experiments showed that resistant transgenic plants could be obtained with any of the constructs, suggesting that the mechanism of protection is independent of the expression of protein and is RNA mediated. Field trial infection confirmed that resistant transgenic events were obtained. Biolistic transient transformation experiments of leaves derived from transgenic plants using a gene coding for the fusion protein GUS-ORF2b, followed by scoring of the number of GUS expressing leaf spots, supported that the protection is mediated by a post-transcriptional gene silencing mechanism.

DOI: 10.1007/s007050170095
PubMed: 11556710

Affiliations:

Argentine

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000864
to stream Main, to step Curation: 000864

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Transgenic resistance in potato plants expressing potato leaf roll virus (PLRV) replicase gene sequences is RNA-mediated and suggests the involvement of post-transcriptional gene silencing.</title>
<author><name sortKey="Vazquez Rovere, C" sort="Vazquez Rovere, C" uniqKey="Vazquez Rovere C" first="C" last="Vazquez Rovere">C. Vazquez Rovere</name>
<affiliation wicri:level="1"><nlm:affiliation>Instituto de Biotecnología, CICVyA CNIA, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina.</nlm:affiliation>
<country xml:lang="fr">Argentine</country>
<wicri:regionArea>Instituto de Biotecnología, CICVyA CNIA, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires</wicri:regionArea>
<wicri:noRegion>Buenos Aires</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Asurmendi, S" sort="Asurmendi, S" uniqKey="Asurmendi S" first="S" last="Asurmendi">S. Asurmendi</name>
</author>
<author><name sortKey="Hopp, H E" sort="Hopp, H E" uniqKey="Hopp H" first="H E" last="Hopp">H E Hopp</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2001">2001</date>
<idno type="RBID">pubmed:11556710</idno>
<idno type="pmid">11556710</idno>
<idno type="doi">10.1007/s007050170095</idno>
<idno type="wicri:Area/Main/Corpus">000864</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000864</idno>
<idno type="wicri:Area/Main/Curation">000864</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000864</idno>
<idno type="wicri:Area/Main/Exploration">000864</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Transgenic resistance in potato plants expressing potato leaf roll virus (PLRV) replicase gene sequences is RNA-mediated and suggests the involvement of post-transcriptional gene silencing.</title>
<author><name sortKey="Vazquez Rovere, C" sort="Vazquez Rovere, C" uniqKey="Vazquez Rovere C" first="C" last="Vazquez Rovere">C. Vazquez Rovere</name>
<affiliation wicri:level="1"><nlm:affiliation>Instituto de Biotecnología, CICVyA CNIA, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina.</nlm:affiliation>
<country xml:lang="fr">Argentine</country>
<wicri:regionArea>Instituto de Biotecnología, CICVyA CNIA, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires</wicri:regionArea>
<wicri:noRegion>Buenos Aires</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Asurmendi, S" sort="Asurmendi, S" uniqKey="Asurmendi S" first="S" last="Asurmendi">S. Asurmendi</name>
</author>
<author><name sortKey="Hopp, H E" sort="Hopp, H E" uniqKey="Hopp H" first="H E" last="Hopp">H E Hopp</name>
</author>
</analytic>
<series><title level="j">Archives of virology</title>
<idno type="ISSN">0304-8608</idno>
<imprint><date when="2001" type="published">2001</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cloning, Molecular (MeSH)</term>
<term>Gene Silencing (MeSH)</term>
<term>Luteovirus (enzymology)</term>
<term>Luteovirus (genetics)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Open Reading Frames (MeSH)</term>
<term>Plants, Genetically Modified (virology)</term>
<term>RNA Replicase (genetics)</term>
<term>Sequence Homology, Nucleic Acid (MeSH)</term>
<term>Solanum tuberosum (genetics)</term>
<term>Solanum tuberosum (virology)</term>
<term>Transformation, Genetic (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Cadres ouverts de lecture (MeSH)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Extinction de l'expression des gènes (MeSH)</term>
<term>Luteovirus (enzymologie)</term>
<term>Luteovirus (génétique)</term>
<term>RNA replicase (génétique)</term>
<term>Similitude de séquences d'acides nucléiques (MeSH)</term>
<term>Solanum tuberosum (génétique)</term>
<term>Solanum tuberosum (virologie)</term>
<term>Transformation génétique (MeSH)</term>
<term>Végétaux génétiquement modifiés (virologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA Replicase</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Luteovirus</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Luteovirus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Luteovirus</term>
<term>Solanum tuberosum</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Luteovirus</term>
<term>RNA replicase</term>
<term>Solanum tuberosum</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Solanum tuberosum</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Plants, Genetically Modified</term>
<term>Solanum tuberosum</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term>
<term>Gene Silencing</term>
<term>Molecular Sequence Data</term>
<term>Open Reading Frames</term>
<term>Sequence Homology, Nucleic Acid</term>
<term>Transformation, Genetic</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Cadres ouverts de lecture</term>
<term>Clonage moléculaire</term>
<term>Données de séquences moléculaires</term>
<term>Extinction de l'expression des gènes</term>
<term>Similitude de séquences d'acides nucléiques</term>
<term>Transformation génétique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Genetically engineered expression of replicase encoding sequences has been proposed as an efficient system to confer protection against virus diseases by eliciting protection mechanisms in the plant. Potato leaf-roll was one of the first diseases for which this kind of protection was engineered in potato plants. However, details of the protecting mechanism were not reported, so far. The ORF2b of an Argentinean strain of PLRV was cloned and sequenced finding 94% and 97% of homology with Australian and Dutch strains, respectively. To elucidate the mechanism of protection against PLRV infection, three versions of ORF2b (non-translatable sense, translatable sense with an engineered ATG and antisense) were constructed under the control of the 35S CaMV promoter and the nos terminator and introduced in potato plants (cv. Kennebec) by Agrobacterium tumefaciens-mediated transformation. Grafting infection experiments showed that resistant transgenic plants could be obtained with any of the constructs, suggesting that the mechanism of protection is independent of the expression of protein and is RNA mediated. Field trial infection confirmed that resistant transgenic events were obtained. Biolistic transient transformation experiments of leaves derived from transgenic plants using a gene coding for the fusion protein GUS-ORF2b, followed by scoring of the number of GUS expressing leaf spots, supported that the protection is mediated by a post-transcriptional gene silencing mechanism.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11556710</PMID>
<DateCompleted><Year>2001</Year>
<Month>09</Month>
<Day>27</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>08</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0304-8608</ISSN>
<JournalIssue CitedMedium="Print"><Volume>146</Volume>
<Issue>7</Issue>
<PubDate><Year>2001</Year>
<Month>Jul</Month>
</PubDate>
</JournalIssue>
<Title>Archives of virology</Title>
<ISOAbbreviation>Arch Virol</ISOAbbreviation>
</Journal>
<ArticleTitle>Transgenic resistance in potato plants expressing potato leaf roll virus (PLRV) replicase gene sequences is RNA-mediated and suggests the involvement of post-transcriptional gene silencing.</ArticleTitle>
<Pagination><MedlinePgn>1337-53</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Genetically engineered expression of replicase encoding sequences has been proposed as an efficient system to confer protection against virus diseases by eliciting protection mechanisms in the plant. Potato leaf-roll was one of the first diseases for which this kind of protection was engineered in potato plants. However, details of the protecting mechanism were not reported, so far. The ORF2b of an Argentinean strain of PLRV was cloned and sequenced finding 94% and 97% of homology with Australian and Dutch strains, respectively. To elucidate the mechanism of protection against PLRV infection, three versions of ORF2b (non-translatable sense, translatable sense with an engineered ATG and antisense) were constructed under the control of the 35S CaMV promoter and the nos terminator and introduced in potato plants (cv. Kennebec) by Agrobacterium tumefaciens-mediated transformation. Grafting infection experiments showed that resistant transgenic plants could be obtained with any of the constructs, suggesting that the mechanism of protection is independent of the expression of protein and is RNA mediated. Field trial infection confirmed that resistant transgenic events were obtained. Biolistic transient transformation experiments of leaves derived from transgenic plants using a gene coding for the fusion protein GUS-ORF2b, followed by scoring of the number of GUS expressing leaf spots, supported that the protection is mediated by a post-transcriptional gene silencing mechanism.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vazquez Rovere</LastName>
<ForeName>C</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Instituto de Biotecnología, CICVyA CNIA, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Asurmendi</LastName>
<ForeName>S</ForeName>
<Initials>S</Initials>
</Author>
<Author ValidYN="Y"><LastName>Hopp</LastName>
<ForeName>H E</ForeName>
<Initials>HE</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName>
<AccessionNumberList><AccessionNumber>AF220151</AccessionNumber>
</AccessionNumberList>
</DataBank>
</DataBankList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>Austria</Country>
<MedlineTA>Arch Virol</MedlineTA>
<NlmUniqueID>7506870</NlmUniqueID>
<ISSNLinking>0304-8608</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>EC 2.7.7.48</RegistryNumber>
<NameOfSubstance UI="D012324">RNA Replicase</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020868" MajorTopicYN="Y">Gene Silencing</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017793" MajorTopicYN="N">Luteovirus</DescriptorName>
<QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016366" MajorTopicYN="N">Open Reading Frames</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012324" MajorTopicYN="N">RNA Replicase</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012689" MajorTopicYN="N">Sequence Homology, Nucleic Acid</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2001</Year>
<Month>9</Month>
<Day>15</Day>
<Hour>10</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2001</Year>
<Month>9</Month>
<Day>28</Day>
<Hour>10</Hour>
<Minute>1</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2001</Year>
<Month>9</Month>
<Day>15</Day>
<Hour>10</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">11556710</ArticleId>
<ArticleId IdType="doi">10.1007/s007050170095</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Argentine</li>
</country>
</list>
<tree><noCountry><name sortKey="Asurmendi, S" sort="Asurmendi, S" uniqKey="Asurmendi S" first="S" last="Asurmendi">S. Asurmendi</name>
<name sortKey="Hopp, H E" sort="Hopp, H E" uniqKey="Hopp H" first="H E" last="Hopp">H E Hopp</name>
</noCountry>
<country name="Argentine"><noRegion><name sortKey="Vazquez Rovere, C" sort="Vazquez Rovere, C" uniqKey="Vazquez Rovere C" first="C" last="Vazquez Rovere">C. Vazquez Rovere</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/AgrobacTransV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000858 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000858 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    AgrobacTransV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:11556710
   |texte=   Transgenic resistance in potato plants expressing potato leaf roll virus (PLRV) replicase gene sequences is RNA-mediated and suggests the involvement of post-transcriptional gene silencing.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:11556710" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a AgrobacTransV1

This area was generated with Dilib version V0.6.38.
Data generation: Fri Nov 20 15:45:55 2020. Site generation: Wed Mar 6 15:24:41 2024

	Serveur d'exploration sur l'agrobacterium et la transgénèse
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'agrobacterium et la transgénèse

Transgenic resistance in potato plants expressing potato leaf roll virus (PLRV) replicase gene sequences is RNA-mediated and suggests the involvement of post-transcriptional gene silencing.

Transgenic resistance in potato plants expressing potato leaf roll virus (PLRV) replicase gene sequences is RNA-mediated and suggests the involvement of post-transcriptional gene silencing.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki